Detection of MERS-CoV antigen on formalin-fixed paraffin-embedded nasal tissue of alpacas by immunohistochemistry using human monoclonal antibodies directed against different epitopes of the spike protein
Identifieur interne : 000629 ( Main/Exploration ); précédent : 000628; suivant : 000630Detection of MERS-CoV antigen on formalin-fixed paraffin-embedded nasal tissue of alpacas by immunohistochemistry using human monoclonal antibodies directed against different epitopes of the spike protein
Auteurs : Ann-Kathrin Haverkamp [Allemagne] ; Berend J. Bosch [Pays-Bas] ; Ingo Spitzbarth [Allemagne] ; Annika Lehmbecker [Allemagne] ; Nigeer Te [Espagne] ; Albert Bensaid [Espagne] ; Joaquim Segalés [Espagne] ; Wolfgang Baumg Rtner [Allemagne]Source :
- Veterinary Immunology and Immunopathology [ 0165-2427 ] ; 2019.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (immunologie), Anticorps monoclonaux (immunologie), Antigènes viraux (immunologie), Camélidés du Nouveau Monde (virologie), Coronavirus du syndrome respiratoire du Moyen-Orient (), Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie), Formaldéhyde, Glycoprotéine de spicule des coronavirus (immunologie), Humains, Immunohistochimie, Inclusion en paraffine, Nez (virologie), Épitopes (immunologie), Études prospectives.
- MESH :
- immunologie : Anticorps antiviraux, Anticorps monoclonaux, Antigènes viraux, Coronavirus du syndrome respiratoire du Moyen-Orient, Glycoprotéine de spicule des coronavirus, Épitopes.
- virologie : Camélidés du Nouveau Monde, Nez.
- Animaux, Coronavirus du syndrome respiratoire du Moyen-Orient, Formaldéhyde, Humains, Immunohistochimie, Inclusion en paraffine, Études prospectives.
English descriptors
- KwdEn :
- Animals, Antibodies, Monoclonal (immunology), Antibodies, Viral (immunology), Antigens, Viral (immunology), Camelids, New World (virology), Epitopes (immunology), Formaldehyde, Humans, Immunohistochemistry, Middle East Respiratory Syndrome Coronavirus (chemistry), Middle East Respiratory Syndrome Coronavirus (immunology), Nose (virology), Paraffin Embedding, Prospective Studies, Spike Glycoprotein, Coronavirus (immunology).
- MESH :
- chemical , immunology : Antibodies, Monoclonal, Antibodies, Viral, Antigens, Viral, Epitopes, Spike Glycoprotein, Coronavirus.
- chemistry : Middle East Respiratory Syndrome Coronavirus.
- immunology : Middle East Respiratory Syndrome Coronavirus.
- virology : Camelids, New World, Nose.
- Animals, Formaldehyde, Humans, Immunohistochemistry, Paraffin Embedding, Prospective Studies.
Abstract
Middle East respiratory syndrome (MERS) represents an important respiratory disease accompanied by lethal outcome in one third of human patients. In recent years, several investigators developed protective antibodies which could be used as prophylaxis in prospective human epidemics. In the current study, eight human monoclonal antibodies (mAbs) with neutralizing and non-neutralizing capabilities, directed against different epitopes of the MERS-coronavirus (MERS-CoV) spike (MERS-S) protein, were investigated with regard to their ability to immunohistochemically detect respective epitopes on formalin-fixed paraffin-embedded (FFPE) nasal tissue sections of MERS-CoV experimentally infected alpacas. The most intense immunoreaction was detected using a neutralizing antibody directed against the receptor binding domain S1B of the MERS-S protein, which produced an immunosignal in the cytoplasm of ciliated respiratory epithelium and along the apical membranous region. A similar staining was obtained by two other mAbs which recognize the sialic acid-binding domain and the ectodomain of the membrane fusion subunit S2, respectively. Five mAbs lacked immunoreactivity for MERS-CoV antigen on FFPE tissue, even though they belong, at least in part, to the same epitope group. In summary, three tested human mAbs demonstrated capacity for detection of MERS-CoV antigen on FFPE samples and may be implemented in double or triple immunohistochemical methods.
Url:
DOI: 10.1016/j.vetimm.2019.109939
PubMed: 31526954
PubMed Central: 7112921
Affiliations:
- Allemagne, Espagne, Pays-Bas
- Basse-Saxe, Catalogne, Utrecht (province)
- Barcelone, Hanovre, Utrecht
- Université autonome de Barcelone, Université d'Utrecht
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Le document en format XML
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Bosch</name><affiliation wicri:level="4"><nlm:aff id="aff0010">Virology Division, Department of Infectious Diseases & Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, the Netherlands</nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Virology Division, Department of Infectious Diseases & Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht</wicri:regionArea><orgName type="university">Université d'Utrecht</orgName><placeName><settlement type="city">Utrecht</settlement><region nuts="2">Utrecht (province)</region></placeName></affiliation></author><author><name sortKey="Spitzbarth, Ingo" sort="Spitzbarth, Ingo" uniqKey="Spitzbarth I" first="Ingo" last="Spitzbarth">Ingo Spitzbarth</name><affiliation wicri:level="3"><nlm:aff id="aff0005">Department of Pathology, University of Veterinary Medicine Hannover Foundation, 30559 Hannover, Germany</nlm:aff><country 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xml:lang="fr">Espagne</country><wicri:regionArea>IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra</wicri:regionArea><placeName><region nuts="2" type="communauté">Catalogne</region><settlement type="city">Barcelone</settlement></placeName><orgName type="university">Université autonome de Barcelone</orgName></affiliation></author><author><name sortKey="Bensaid, Albert" sort="Bensaid, Albert" uniqKey="Bensaid A" first="Albert" last="Bensaid">Albert Bensaid</name><affiliation wicri:level="4"><nlm:aff id="aff0020">IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra</wicri:regionArea><placeName><region nuts="2" type="communauté">Catalogne</region><settlement type="city">Barcelone</settlement></placeName><orgName type="university">Université autonome de Barcelone</orgName></affiliation></author><author><name sortKey="Segales, Joaquim" sort="Segales, Joaquim" uniqKey="Segales J" first="Joaquim" last="Segalés">Joaquim Segalés</name><affiliation wicri:level="3"><nlm:aff id="aff0025">Departament de Sanitat i Anatomia Animals, Facultat de Veterinària, UAB, 08193 Bellaterra, Barcelona, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Departament de Sanitat i Anatomia Animals, Facultat de Veterinària, UAB, 08193 Bellaterra, Barcelona</wicri:regionArea><placeName><settlement type="city">Barcelone</settlement><region nuts="2" type="region">Catalogne</region></placeName></affiliation><affiliation wicri:level="4"><nlm:aff id="aff0030">UAB, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>UAB, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra</wicri:regionArea><placeName><region nuts="2" type="communauté">Catalogne</region><settlement type="city">Barcelone</settlement></placeName><orgName type="university">Université autonome de Barcelone</orgName></affiliation></author><author><name sortKey="Baumg Rtner, Wolfgang" sort="Baumg Rtner, Wolfgang" uniqKey="Baumg Rtner W" first="Wolfgang" last="Baumg Rtner">Wolfgang Baumg Rtner</name><affiliation wicri:level="3"><nlm:aff id="aff0005">Department of Pathology, University of Veterinary Medicine Hannover Foundation, 30559 Hannover, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Pathology, University of Veterinary Medicine Hannover Foundation, 30559 Hannover</wicri:regionArea><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Hanovre</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:aff id="aff0015">Center for Systems Neuroscience, 30559 Hannover, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Center for Systems Neuroscience, 30559 Hannover</wicri:regionArea><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Hanovre</settlement></placeName></affiliation></author></analytic><series><title level="j">Veterinary Immunology and Immunopathology</title><idno type="ISSN">0165-2427</idno><idno type="eISSN">1873-2534</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Monoclonal (immunology)</term><term>Antibodies, Viral (immunology)</term><term>Antigens, Viral (immunology)</term><term>Camelids, New World (virology)</term><term>Epitopes (immunology)</term><term>Formaldehyde</term><term>Humans</term><term>Immunohistochemistry</term><term>Middle East Respiratory Syndrome Coronavirus (chemistry)</term><term>Middle East Respiratory Syndrome Coronavirus (immunology)</term><term>Nose (virology)</term><term>Paraffin Embedding</term><term>Prospective Studies</term><term>Spike Glycoprotein, Coronavirus (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux (immunologie)</term><term>Anticorps monoclonaux (immunologie)</term><term>Antigènes viraux (immunologie)</term><term>Camélidés du Nouveau Monde (virologie)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient ()</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie)</term><term>Formaldéhyde</term><term>Glycoprotéine de spicule des coronavirus (immunologie)</term><term>Humains</term><term>Immunohistochimie</term><term>Inclusion en paraffine</term><term>Nez (virologie)</term><term>Épitopes (immunologie)</term><term>Études prospectives</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Antibodies, Viral</term><term>Antigens, Viral</term><term>Epitopes</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term><term>Anticorps monoclonaux</term><term>Antigènes viraux</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Glycoprotéine de spicule des coronavirus</term><term>Épitopes</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Camélidés du Nouveau Monde</term><term>Nez</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Camelids, New World</term><term>Nose</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Formaldehyde</term><term>Humans</term><term>Immunohistochemistry</term><term>Paraffin Embedding</term><term>Prospective Studies</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Formaldéhyde</term><term>Humains</term><term>Immunohistochimie</term><term>Inclusion en paraffine</term><term>Études prospectives</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Middle East respiratory syndrome (MERS) represents an important respiratory disease accompanied by lethal outcome in one third of human patients. In recent years, several investigators developed protective antibodies which could be used as prophylaxis in prospective human epidemics. In the current study, eight human monoclonal antibodies (mAbs) with neutralizing and non-neutralizing capabilities, directed against different epitopes of the MERS-coronavirus (MERS-CoV) spike (MERS-S) protein, were investigated with regard to their ability to immunohistochemically detect respective epitopes on formalin-fixed paraffin-embedded (FFPE) nasal tissue sections of MERS-CoV experimentally infected alpacas. The most intense immunoreaction was detected using a neutralizing antibody directed against the receptor binding domain S1B of the MERS-S protein, which produced an immunosignal in the cytoplasm of ciliated respiratory epithelium and along the apical membranous region. A similar staining was obtained by two other mAbs which recognize the sialic acid-binding domain and the ectodomain of the membrane fusion subunit S2, respectively. Five mAbs lacked immunoreactivity for MERS-CoV antigen on FFPE tissue, even though they belong, at least in part, to the same epitope group. In summary, three tested human mAbs demonstrated capacity for detection of MERS-CoV antigen on FFPE samples and may be implemented in double or triple immunohistochemical methods.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Adney, D R" uniqKey="Adney D">D.R. Adney</name></author><author><name sortKey="Bielefeldt Ohmann, H" uniqKey="Bielefeldt Ohmann H">H. Bielefeldt-Ohmann</name></author><author><name sortKey="Hartwig, A E" uniqKey="Hartwig A">A.E. Hartwig</name></author><author><name sortKey="Bowen, R A" uniqKey="Bowen R">R.A. Bowen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Adney, D R" uniqKey="Adney D">D.R. Adney</name></author><author><name sortKey="Brown, V R" uniqKey="Brown V">V.R. 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Enjuanes</name></author><author><name sortKey="Drabek, D" uniqKey="Drabek D">D. Drabek</name></author><author><name sortKey="Bosch, B J" uniqKey="Bosch B">B.-J. Bosch</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zaki, A M" uniqKey="Zaki A">A.M. Zaki</name></author><author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S. van Boheemen</name></author><author><name sortKey="Bestebroer, T M" uniqKey="Bestebroer T">T.M. Bestebroer</name></author><author><name sortKey="Osterhaus, A D" uniqKey="Osterhaus A">A.D. Osterhaus</name></author><author><name sortKey="Fouchier, R A" uniqKey="Fouchier R">R.A. Fouchier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zumla, A" uniqKey="Zumla A">A. Zumla</name></author><author><name sortKey="Hui, D S" uniqKey="Hui D">D.S. Hui</name></author><author><name sortKey="Perlman, S" uniqKey="Perlman S">S. Perlman</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Allemagne</li><li>Espagne</li><li>Pays-Bas</li></country><region><li>Basse-Saxe</li><li>Catalogne</li><li>Utrecht (province)</li></region><settlement><li>Barcelone</li><li>Hanovre</li><li>Utrecht</li></settlement><orgName><li>Université autonome de Barcelone</li><li>Université d'Utrecht</li></orgName></list><tree><country name="Allemagne"><region name="Basse-Saxe"><name sortKey="Haverkamp, Ann Kathrin" sort="Haverkamp, Ann Kathrin" uniqKey="Haverkamp A" first="Ann-Kathrin" last="Haverkamp">Ann-Kathrin Haverkamp</name></region><name sortKey="Baumg Rtner, Wolfgang" sort="Baumg Rtner, Wolfgang" uniqKey="Baumg Rtner W" first="Wolfgang" last="Baumg Rtner">Wolfgang Baumg Rtner</name><name sortKey="Baumg Rtner, Wolfgang" sort="Baumg Rtner, Wolfgang" uniqKey="Baumg Rtner W" first="Wolfgang" last="Baumg Rtner">Wolfgang Baumg Rtner</name><name sortKey="Lehmbecker, Annika" sort="Lehmbecker, Annika" uniqKey="Lehmbecker A" first="Annika" last="Lehmbecker">Annika Lehmbecker</name><name sortKey="Lehmbecker, Annika" sort="Lehmbecker, Annika" uniqKey="Lehmbecker A" first="Annika" last="Lehmbecker">Annika Lehmbecker</name><name sortKey="Spitzbarth, Ingo" sort="Spitzbarth, Ingo" uniqKey="Spitzbarth I" first="Ingo" last="Spitzbarth">Ingo Spitzbarth</name><name sortKey="Spitzbarth, Ingo" sort="Spitzbarth, Ingo" uniqKey="Spitzbarth I" first="Ingo" last="Spitzbarth">Ingo Spitzbarth</name></country><country name="Pays-Bas"><region name="Utrecht (province)"><name sortKey="Bosch, Berend J" sort="Bosch, Berend J" uniqKey="Bosch B" first="Berend J." last="Bosch">Berend J. Bosch</name></region></country><country name="Espagne"><region name="Catalogne"><name sortKey="Te, Nigeer" sort="Te, Nigeer" uniqKey="Te N" first="Nigeer" last="Te">Nigeer Te</name></region><name sortKey="Bensaid, Albert" sort="Bensaid, Albert" uniqKey="Bensaid A" first="Albert" last="Bensaid">Albert Bensaid</name><name sortKey="Segales, Joaquim" sort="Segales, Joaquim" uniqKey="Segales J" first="Joaquim" last="Segalés">Joaquim Segalés</name><name sortKey="Segales, Joaquim" sort="Segales, Joaquim" uniqKey="Segales J" first="Joaquim" last="Segalés">Joaquim Segalés</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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